Rapid procedure for chemical sequencing of small oligonucleotides without ethanol precipitation

a novel technique of small incision fascia lata harvesting without a faciatome for frontalis suspension procedure

purpose : to introduce a small incision technique of fascia lata (fl) harvesting for frontalis suspension blepharoptosis procedure. methods : a skin incision was made in a line between the lateral condyle of the tibia and the anterior superior iliac crest, starting 4- 5 cm above the knee and extending upward 2- 2.5 cm . approximately 8 cm superior to the first incision, a second skin incision w...

Chemical Synthesis of Oligonucleotides

It can fairly be said that synthetic oligonucleotides are the fuel that drives the engine of molecular biology. Nearly every technique in use today in molecular biology employs chemically synthesized DNAs or RNAs. This includes PCR, Real-Time PCR, DNA sequencing, site directed mutagenesis, single-nucleotide polymorphism (SNP) assays, microarrays, and the rapidly expanding world of small RNAs. U...

Small-scale mashing procedure for predicting ethanol yield of sorghum grain

A small-scale mashing (SSM) procedure requiring only 300 mg of samples was investigated as a possible method of predicting ethanol yield of sorghum grain. The initial SSM procedure, which was conducted similarly to the mashing step in a traditional fermentation test, hydrolyzed just 38.5–47.2% of total sorghum starch to glucose. The initial procedure was simplified to contain only one liquefact...

A Procedure for Preparation of Semi-activated Carbon Fiber without any Treatment under High Temperature

A procedure for preparation of semi-activated carbon fiber (SACF) without any treatment under high temperature was proposed. The first step of the procedure is coating of an inorganic fiber (E-glass fiber) by an adsorbent mixture including powder activated carbon, methyl cellulose and water. In this work a set of experiments was performed to attain appropriate adsorbent mixture for good quality...

Chemical Synthesis and Purification of Oligonucleotides